| Title | Fatigue and stress studies : an improved semi-automated procedure for flurometric determination of plasma catecholamines. |
|---|---|
| Record ID | 38936 |
| Personal Name Creator |
Fiorica, Vincent |
| Corporate Creator | United States. Office of Aviation Medicine |
| Publisher | Civil Aerospace Medical Institute |
| Publication Date | 19660400 |
| Language | English |
| Abstract | A semiautomated technique is described for the estimation of total catecholamines in plasma by the trihydroxyindole procedure. The method utilizes conventional alumina-column chromatography for isolation of the amines. Catecholamine oxidation, tautomerization, and fluorescence detection are accomplished by an automated chemical system. Recoveries from plasma of 0.01 and 0.02 micrograms total catecholamines/10-ml sample were 86.4% plus or minus 8.9% (SD) and 85.6% plus or minus 5.8% (SD), respectively. Total catecholamine level in resting human plasma, determined by the present technique, was 0.89 plus or minus 0.15 (SD) micrograms/l. |
| Rosap ID | dot:20766 |
| Rosap URL | https://rosap.ntl.bts.gov/view/dot/20766 |
| TRT Terms | Automation; Chemical analysis; Amines; Reagents; Chemical reactions; Alumina; Sodium; Acetates; Acetic acid; Sodium hydroxide; Fluorescence; Blood analysis; Absorption; Human subject testing; Physiological fatigue; Physiological stress |
| General Subjects | Automation; Chemistry, Analytic; Catecholamines; Chemical tests and reagents; Chemical reactions; Blood plasma; Blood--Analysis; Aluminum oxide; Fluorimetry; Sodium acetate; Acetic acid; Ethylenediaminetetraacetic acid; Vitamin C; Sodium hydroxide; Perchloric acid; Ferrocyanides; Absorption; Fatigue; Stress (Physiology) |
| Classification | NTL - AVIATION - AVIATION |
| Geographical Coverage |
United States |
| Report Number | AM 66-6; DOT/FAA/AM-66/06 |
| Resource type | Tech Report |
| URL | https://ntlrepository.blob.core.windows.net/lib/38000/38900/38936/AM66-06.pdf |
| Format | |
| Database | NTL Digital Repository |