| Title | Automated differential fluorometric analysis of norepinephrine and epinephrine in blood plasma and urine. |
|---|---|
| Record ID | 39126 |
| Personal Name Creator |
Fiorica, Vincent; Moses, Russell |
| Corporate Creator | Civil Aeromedical Institute |
| Corporate Contributor |
United States. Office of Aviation Medicine |
| Publisher | Civil Aerospace Medical Institute |
| Publication Date | 19710400 |
| Language | English |
| Abstract | An automated fluorometric trihydroxyindole procedure is described for the measurement of norepinephrine (NE) and epinephrine (E) in blood plasma or urine. The method employs conventional techniques for isolation of the catecholamines by alumina column chromatography. Column eluates are analyzed in an AutoAnalyzer system incorporating two fluorometers. Differentiation of the amines is based on differences in the fluorescence characteristics of the lutines of NE and E. The accuracy (as estimated by recoveries of added amines) and precision of the method are comparable to those reported for other trihydroxyindole techniques. Representative values for urinary excretion and for plasma levels of NE and E compare favorably with values stated in the literature. |
| Rosap ID | dot:20935 |
| Rosap URL | https://rosap.ntl.bts.gov/view/dot/20935 |
| TRT Terms | Aviation medicine; Blood analysis; Urine; Fluorometers; Physiological stress; Physiological fatigue; Chromatography; Chemical analysis; Chemical solutions; Mixtures; Reagents; Distillation; Centrifugal force; Electromagnetic waves by wavelength; Light; Hydrogen; Sodium; Oxygen; Chlorine; Acetic acid; Urea; Water; Iron compounds; Hydroxides; Chlorine compounds; Carbon; Nitrogen; Chemical compounds; Inorganic compounds; Organic compounds; Amines; Oxidation; Measurement; Standard deviation; Human subject testing |
| General Subjects | Aviation medicine; Blood plasma--Analysis; Urine--Analysis; Fluorimetry; Fluorimeter; Stress (Physiology); Fatigue; Chromatographic analysis; Chemistry, Analytic--Automation; Solution (Chemistry); Mixtures; Distillation; Distillation apparatus; Chemical tests and reagents; Catecholamines; Adrenaline; Noradrenaline; Thioglycolic acid; Sodium acetate; Ethylenediamine; Acetic acid; Electromagnetic waves; Light; Centrifuges; Oxidation; Hydroxides; Hydrogen; Chlorides; Ferrocyanides; Inorganic compounds; Organic compounds |
| Classification | NTL - AVIATION - AVIATION; NTL - AVIATION - Aviation Human Factors |
| Geographical Coverage |
United States |
| Report Number | FAA-AM-71-15; DOT/FAA/AM-71/15 |
| Resource type | Tech Report |
| URL | https://ntlrepository.blob.core.windows.net/lib/39000/39100/39126/AM71-15.pdf |
| Format | |
| Database | NTL Digital Repository |