| Title | A Rapid and Inexpensive PCR-Based STR Genotyping Method for Identifying Forensic Specimens |
|---|---|
| Record ID | 39970 |
| Personal Name Creator |
Kupfer, D. M.; Cassidy, Brandt; Vu, Nicole; Burian, Dennis; Canfield, Dennis V.; Huggins, Mark |
| Corporate Creator | Advancia Corporation; DNA Solutions |
| Corporate Contributor |
Federal Aviation Administration |
| Publisher | United States. Department of Transportation. Federal Aviation Administration |
| Publication Date | 20060600 |
| Language | English |
| Abstract | Situations arise where forensic samples can be inadvertently misidentified during field collection. Samples identified in the field as being from the same person that return conflicting toxicological results may suggest such misidentification. Polymerase chain reaction (PCR)-based human identity testing provides a reliable and independent method to confirm sample identification. In the study presented here, the Federal Bureau of Investigation’s human Combined DNA Identity System (CODIS) loci were used on a rapid, inexpensive microfluidics chip electrophoresis platform to confirm the identity of forensic samples from an aircraft accident site. Absolute allele identification was not achieved with this method, but it was found to be suitable for comparative analysis, as demonstrated by validation of the results and conclusions from capillary electrophoresis. The nine CODIS Short Tandem Repeat loci and a gender discrimination locus used in this study have a greater than 5E+07 matching probability suitable for small sample sizes. |
| Rosap ID | dot:40488 |
| Rosap URL | https://rosap.ntl.bts.gov/view/dot/40488 |
| TRT Terms | Air transportation crashes; Forensic medicine; Forensic science; Specimens; Toxicology |
| General Subjects | Aviation; Safety and Human Factors; DNA Analysis; Human beings |
| Geographical Coverage |
United States |
| TRIS Online Accession No |
1032974 |
| Report Number | DOT/FAA/AM-06/14 |
| Resource type | Tech Report |
| URL | https://ntlrepository.blob.core.windows.net/lib/39000/39900/39970/200614.pdf |
| Format | |
| Database | NTL Digital Repository |