| Title | Carbamazepine Positives Due To 10,11-Dihydro-10-Hydroxycarbamazepine Breakdown in the GC/MS Injector Port |
|---|---|
| Record ID | 81790 |
| Personal Name Creator |
Johnson, Robert D.; Lewis, Russell J.; Angier, Mike K. |
| Corporate Creator | United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute |
| Corporate Contributor |
United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Office of Aerospace Medicine |
| Publisher | United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute |
| Publication Date | 20100201 |
| Language | English |
| Abstract | During the investigation of aviation accidents, postmortem specimens from accident victims are submitted to the Federal Aviation Administration’s Civil Aerospace Medical Institute (CAMI) for toxicological analysis. A case recently received by CAMI screened positive for the anticonvulsant medication carbamazepine (Tegretol® ) by gas chromatography/mass spectrometry (GC/MS). The carbamazepine found during the routine screening procedure was subsequently confirmed using a carbamazepine-specific GC/MS procedure. Concurrently, it was discovered that the accident victim had been prescribed oxcarbazepine (Trileptal® ). Oxcarbazepine is nearly structurally identical to carbamazepine and is metabolized by cytosolic enzymes in the liver to the active compound 10,11- dihydro-10-hydroxycarbamazepine. The carbamazepine initially found in this case was present due to the breakdown of the active oxcarbazepine metabolite in the GC/MS injector port. In the current study this conversion is investigated, the percentage of carbamazepine formed at various injector port temperatures is determined, and these three compounds are quantified in nine fluid and tissue specimens from the case in question. Lastly, liquid chromatography/mass spectrometry (LC/MS) was used to demonstrate the absence of carbamazepine, and its formation, in the same specimens. |
| Public Note | This work was accomplished under the approved task AM-B-05-TOX-204 |
| Rosap ID | dot:57097 |
| Rosap URL | https://rosap.ntl.bts.gov/view/dot/57097 |
| TRT Terms | Forensic science; Toxicology; Crash investigation; Air transportation crashes |
| General Subjects | Forensic toxicology; Oxcarbazepine; Carbamazepine; GC/MS; LC/MS; aircraft accident investigation |
| Geographical Coverage |
United States |
| TRIS Online Accession No |
1155829 |
| Report Number | DOT/FAA/AM-10/4 |
| Resource type | Tech Report |
| URL | https://ntlrepository.blob.core.windows.net/lib/81000/81700/81790/201004.pdf |
| Format | |
| Database | NTL Digital Repository |