| Title | Comparison of Species-Specific β-Glucuronidase Hydrolysis of Cannabinoid Metabolites in Human Urine |
|---|---|
| Record ID | 81841 |
| Personal Name Creator |
Kemp, Philip M.; Cliburn, Kacey D |
| Corporate Creator | United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute |
| Corporate Contributor |
United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Office of Aerospace Medicine |
| Publisher | United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute |
| Publication Date | 20150301 |
| Language | English |
| Abstract | Toxicological investigations of fatal aviation accidents include testing for ∆9 -tetrahydrocannabinol (THC), the active component of marijuana, and its major metabolite, 11-nor-9-carboxy-∆9 -THC (THCCOOH) in postmortem fluids and tissues from deceased pilots. These cannabinoids, as well as others, exist in human urine as their glucuronide conjugates, formed during Phase II metabolism to increase their solubility for excretion. Prior to the analysis of cannabinoids in urine by gas chromatography/mass spectrometry (GC-MS), it is desirable to cleave (hydrolyze) the glucuronic acid to enhance the detection of the unconjugated compounds. In the current study, β-glucuronidases from 3 mollusk species (Helix pomatia, Patella vulgate, and Haliotis rufescens), 1 bacteria (Escherichia coli), and bovine liver were compared for their quantitative efficiency in hydrolyzing the glucuronide bond in spiked, blank urine. |
| Rosap ID | dot:57190 |
| Rosap URL | https://rosap.ntl.bts.gov/view/dot/57190 |
| TRT Terms | Animals; Gas chromatography; Hydrolysis; Marijuana; Mass spectrometry; Toxicology; Urine; Forensic science; Aviation |
| General Subjects | Glucuronidase; Cannabinoids; Tetrahydrocannabinol |
| Geographical Coverage |
United States |
| TRIS Online Accession No |
1563640 |
| Report Number | DOT/FAA/AM-15/6 |
| Resource type | Tech Report |
| URL | https://ntlrepository.blob.core.windows.net/lib/81000/81800/81841/201506.pdf |
| Format | |
| Database | NTL Digital Repository |